Rod to hook transition for extracellular flagellum 4 assembly is catalyzed by the L - ring - dependent rod - 5 scaffold removal
نویسنده
چکیده
Eli J. Cohen and Kelly T. Hughes 8 9 Department of Biology, University of Utah, Salt Lake City, UT 84112, USA 10 11 12 1 Corresponding author: 13 Kelly T. Hughes; Mailing address: Department of Biology, University of Utah, 257 14 South 1400 East, Salt Lake City, UT, 84112, USA; Tel: +801-587-3367; Fax: +80115 585-9735; E-mail: [email protected] 16 17 18 19
منابع مشابه
Rod-to-hook transition for extracellular flagellum assembly is catalyzed by the L-ring-dependent rod scaffold removal.
In Salmonella, the rod substructure of the flagellum is a periplasmic driveshaft that couples the torque generated by the basal body motor to the extracellular hook and filament. The rod subunits self-assemble, spanning the periplasmic space and stopping at the outer membrane when a mature length of ~22 nm is reached. Assembly of the extracellular hook and filament follow rod completion. Hook i...
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The rod component of the bacterial flagellum polymerizes from the inner membrane across the periplasmic space and stops at a length of 25 nm at the outer membrane. Bushing structures, the P- and L-rings, polymerize around the distal rod and form a pore in the outer membrane. The flagellar hook structure is then added to the distal rod growing outside the cell. Hook polymerization stops after th...
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The process of flagellar assembly in Salmonella typhimurium was investigated by using temperature-sensitive mutants. The mutants were grown at the restrictive temperature and then at the permissive temperature, with radiolabel supplied in the first phase of the experiment and not the second, or vice versa. Flagellar hook-basal body complexes were then purified and analyzed by gel electrophoresi...
متن کاملFlgD is a scaffolding protein needed for flagellar hook assembly in Salmonella typhimurium.
FlgD is known to be absolutely required for hook assembly, yet it has not been detected in the mature flagellum. We have overproduced and purified FlgD and raised an antibody against it. By using this antibody, we have detected FlgD in substantial amounts in isolated basal bodies from flgA, flgE, flgH, flgI, flgK, and fliK mutants, in much smaller amounts in those from the wild type and flgL, f...
متن کاملPosttranscriptional control of the Salmonella enterica flagellar hook protein FlgE.
Previous work suggested that the FlgE (flagellar hook subunit) protein in Salmonella enterica serovar Typhimurium was posttranscriptionally regulated in response to the stage of flagellar assembly. Specifically, the FlgE protein could be detected in flagellar mutants defective at the stages of assembly before or after rod assembly but not in rod assembly mutants, yet flgE mRNA levels were unaff...
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تاریخ انتشار 2014